Verified answer

It means you have one part of the substance in question and dilute it in 49 parts of the DNA Polymerase Buffer solution.

So if you use 1 mL of substance, you add 49 mL of solution to dilute it and weaken it/buffer it.

So you don't make solutions in China? It's similar to one part tea, 49 parts water= one damn good cup of tea.:)

"1 in 50" is a dilution factor/dilution ratio, which, in a general sense, you are being ask to make a 1 is to 50 dilution of your initial sample with a diluent (the solution you would use to dilute your sample).

so, if, for example, you want to dilute your DNA sample in a polymerase buffer with a 1/50 dilution, you would use, again as an example, 1 uL (microliter) of your DNA sample and dilute it with 49 uL of polymerase buffer...that should give you a 1 in 50 diluted DNA sample. The volumetric units could change depending on how much you are working on...I assume it's PCR, so that might be in micron volumes.

Hope this helps!

it means mix 1 part in 50 parts eg mix 1mL of ur solution in 50mL distilled water solution