If you are just asking how to make solutions of those concentrations from the solid, let's say you want to make 10 mL of 2 micromolar cytochalasin D:
0.01 L X 2X10^-6 mol/L X 508 g/mol = 1.02 X 10^-5 grams
Obviously you cannot weigh out that small an amount. So, you'll need to make a much more concentrated stock solution and then dilute it. So, if the compound is sufficiently soluble, I would make 10 mL of a 2 mM stock solution first:
0.010 L X 2 X 10^-3 mol/L X 508 g/mol = 0.0102 g = 10.2 mg
So, weigh out 10.2 mg of the compound and dissolve it in 10 mL of buffer or water.
Then to produce 10 mL of the 2 micromolar solution:
This is a 1:1000 dilution of the 2 mM stock. So, you could take 10 microliters of that stock and add it to 10 mL of water or buffer. That would produce 10 mL of the 2 micromolar solution.
You could then make a series of dilutions of that 2 micromolar stock to produce other concentrations. Or you could use the 2 mM stock to produce other dilutions.
You can always use the equation M1V1 = M2V2 to calculate the volumes of the stock solution required to produce the desired volume of the correct concentration.
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If you are just asking how to make solutions of those concentrations from the solid, let's say you want to make 10 mL of 2 micromolar cytochalasin D:
0.01 L X 2X10^-6 mol/L X 508 g/mol = 1.02 X 10^-5 grams
Obviously you cannot weigh out that small an amount. So, you'll need to make a much more concentrated stock solution and then dilute it. So, if the compound is sufficiently soluble, I would make 10 mL of a 2 mM stock solution first:
0.010 L X 2 X 10^-3 mol/L X 508 g/mol = 0.0102 g = 10.2 mg
So, weigh out 10.2 mg of the compound and dissolve it in 10 mL of buffer or water.
Then to produce 10 mL of the 2 micromolar solution:
This is a 1:1000 dilution of the 2 mM stock. So, you could take 10 microliters of that stock and add it to 10 mL of water or buffer. That would produce 10 mL of the 2 micromolar solution.
You could then make a series of dilutions of that 2 micromolar stock to produce other concentrations. Or you could use the 2 mM stock to produce other dilutions.
You can always use the equation M1V1 = M2V2 to calculate the volumes of the stock solution required to produce the desired volume of the correct concentration.
Cyto D