Verified answer

We use the equation Π = MRT or Π = iMRT depending on what problem you're solving.

where

Π is the osmotic pressure in atm

i = van 't Hoff factor of the solute.

M = molar concentration in mol/L(which in this case is 6.5×104g/mol)

R = universal gas constant = 15.9mL

T = absolute temperature in K

Step 1: - Determine the van 't Hoff factor

Since glucose does not dissociate into ions in solution, the van 't Hoff factor = 1

Step 2: - Find absolute temperature

T = °C + 273

T = 18 + 273

T = 291 K

Step 3: - Find concentration of hemoglobin

Π = iMRT

M = Π/iRT

M = 16.75 atm/(1)(6.5×104g/mol·K)(291)

The M should be in your notes

Step 4: - Find amount of sucrose per liter

M = mol/Volume

mol = M·Volume

The rest are in your notes as I cannot tell you everything, also, look up the periodic table for the elements, calculate the molar mass of hemoglobin and mass of hemoglobin

Π = i*c*R*T, where Π = osmotic pressure (kPa), c = concentration (M) and T = temp (K)

hemoglobin does not dissociate so i = 1

R = gas constant = 8.314 L-kPa/K-mol

c = 16.75*10^-3/(6.5*10^4*0.0159) = 1.62*10^-5 M

T = 291.15 K

Π = (8.314*1.62*10^-5)*291.15 = 0.0392 kPa = 39.2 Pa