You are doing your rotation in the hospital’s clinical laboratory. A sample of cloudy cerebrospinal fluid (CSF?

B. You are doing your rotation in the hospital’s clinical laboratory. A sample of cloudy cerebrospinal fluid (CSF) from a suspected meningitis case arrives and you are told to Gram stain it, and then to plate it on blood and chocolate agar. In the Gram stain you find Gram negative rods of varying size and shape. You also find a lot of bacteria inside phagocytic cells. They are not diplococci. Colonies grow on both of the plates you inoculated.

Later, the charge nurse tells you that the patient, a 3 year old girl, has not had any childhood vaccinations.

What is the most likely causative organism? Why?

Why was the child’s unvaccinated status helpful in diagnosis?

What is causing the cloudiness of the CSF?

What other types of infections can this organism cause in children?

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